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Doctorate in Veterinary Medicine, Universite de Montréal, Canada
Masters of Science (M.Sc), University of Guelph, Canada
Graduate Courses taken:
Thesis: "Platelet Morphology in Porcine Stress Syndrome".
Director: Dr. P.K. Basrur.
Histological and ultrastructural studies of blood platelets from pigs carrying the mutant gene for malignant hyperthermia (Porcine Stress Syndrome) and positive to the susceptibility to halothane test. A morphological anomaly of platelets has been identified and could be associated with malignant hyperthermia. The impact on platelet morphology of anticoagulants used for blood collection was examined to minimize their influence on the results.
Results have been communicated in scientific meetings and published in scientific journals:
Citations appeared in European Journal of Haematology (Softeland et al. 1992 49:161-173, Porcine Platelets in Vitro and in vivo studies-Relevance to human thrombosis research) and Free Radical Biology and Medicine, (Duthie GG and Arthur JR), 1993: 14 435-442, Free radicals and calcium homeostasis- Relevance to malignant hyperthermia).
PhD in Biomedical Sciences, University of Guelph, Guelph, Canada.
(Qualifying Exam) in 1986: Comparative Mammalian Genetics and Reproduction
Thesis: "Fragile X Chromosome in Baldy Calf Syndrome".
Director: Dr. P.K. Basrur.
Baldy Calf Syndrome, an inherited condition in cattle, was investigated using cytogenetics, moprhological and clinical data. An association of a break in the X chromosome was associated with parakeratosis and reduced immunocompetence leading to death in a few months .Skin defects of genetic origin in cattle were reviewed and comparaison was established with the human fragile X syndrome .
Other studies were conducted in cattle: a case of folic-acid sensitive alopecia in a male Charolais calf ( from a sire-daughter mating) and a family carrying an X-autosome translocation and the gene for albinism.
Results were presented at various international congresses and published in scientific journals.
Citations appeared in American Journal of Human Genetics in a paper by a Dutch group (Deelen et al. 1994, 51:513-516, Conservation of CGG region in FMR1 gene in Mammals) et in Hereditas in another paper by a Danish scientist (Ronne M, 1995, 122:153-162, Localisation of fragile sites in the karyotype of Sus Scrofa Domestica- Present Status).
Short Course in Medical and Mammalian Genetics
The Jackson Laboratory, Bar Harbor, Maine, USA.
A two-weeks intensive course for MDs and researchers from around the world, given by Faculty members from the Department of Genetic,John Hopkins School of Medicine, Baltimore; the research staff of the Jackson Laboratory in Maine, and invited lecturers from around the world.2001-2003
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All my career has been oriented toward Medical Genetics, and the investigation of various animal models for human genetic diseases. Techniques used in human genetics and applied to various animal species ranged from Histology to Molecular Biology, including Electron Microscopy, Cell Culture, Cytogenetics, Flow Cytometry and Bioinformatics.
Post-Doctoral Fellow ( Comparative cytogenetics & reproduction)
Cytogenetics Laboratory, Biotechnology Building, Animal Genetics Department, (INRA) Institut National de la Recherche Agronomique, 78350 Jouy en Josas, France.
Director: Dr. C.P. Popescu. Tel: 01 34 65 26 70.
One-year fellowship awarded by the French Ministeredes Affaires Etrangeres, through the French Ambassy in Canada after a national competition. The aim was to strengthen scientific exchanges between Canada and France in various fields including Biotechnology. This fellowship was renewed for another year.
The investigations undertaken were part of the on-going research program in the Cytogenetics Laboratory on the impact of chromosomal anomalies on reproduction of livestock and their economical consequences. I examined under electron microscopy the behavior of synaptonemal complexes during meiosis, in bulls and boars carrying chromosome translocations.
My interest in comparative reproduction, associated with my experience in electron microscopy and cytogenetics, has permitted an easy integration into this group.
I applied the technique used by Professor Yves Rumpler of the Faculte de Medecine de Strasbourg in humans, to pigs and cattle, in collaboration with the Ecole Veterinaire de Toulouse and the Faculta di Agraria di Milano.
In contrast to the human medical litterature where pubhished papers showed abnormal pairing of translocated chromosomes with the sex chromosomes during meiosis, results of these studies showed that there was no abnormal pairing of translocated chromosomes with sex chromosomes during meiosis and that the fertility problems noted in relation to these sires could be attributed only to abnormal segregation of chromosomes after meiosis
A close scrutiny of the studies done in humans and cattle showed that they belong to two different groups of males with fertility problems. In humans, testicular biopsy is permitted only in extreme cases of oligo or aspermia, and the reduced production of spermatozoids was the results of abnormal chromosomal pairing. In contrast, the animals studied were adult males, already selected for breeding and they came under scrutiny only after they produced a reduced number of offspring. These studies are complementary and should not be attributed to differences between species .
In mouse, published papers showed that depending on the chromosomes involved, a wide range of impact on spermatogenesis was noted. From azoospermia associated with abnormal pairing (as in men) to normal production of semen ( as in livestock).
Result were presented at scientific meetings and published in scientific journals.
Science Citation Index showed an international impact of this work( in 1989 by Gustavsson et al. Cytogenetics and Cell Genetics, 50 188-194; in 1991 par Hansen and Hansen, Genetics Selection Evolution S1:S74-77; in 1992 par Weber et al, Journal of The American Veterinary Association 200: 1216-1219,et by Zhang et al., Theriogenology 37:553-558; and in 1994 by Amaral et Jorge, Genetics Selection, Evolution, 1994:177-185).
During that period, I participated in the International Standardization Meeting on Chromosomes of Domestic Animals held in Jouy en Josas en 1989, as an organizer as well as a delegate.
I became also involved in the gene mapping work in the Cytogenetics Laboratory and I compiled the gene map of cattle:
Higher Scientific Officer (gene mapping, flow cytometry & molecular biology)
Laboratory of Genome Mapping Department of Molecular & Cellular Physiology, Animal Physiology & Genetics Research Institute, Agricultural and Food Research Council, Babraham, Cambridge, UK. CB2 4AT.
My experience in porcine cytogenetics combined with my interest for comparative gene mapping in mammals led to my involvement in the European BRIDGE program PiGMaP (Pig Gene Mapping Project), and in particular the standardization of the porcine flow karyotype.
To accelerate the process of gene mapping to a specific chromosome and the establishment of chromosome-specific DNA libraries, it was necessary to identify each of the chromosomes in the pig flow karyotype to individually sort them using electro-magnetic techniques.
The application of various techniques (cytogenetics, flow cytometry and molecular biology) to porcine peripheral blood lymphocytes and pig-mouse somatic cell hybrids has permitted the first standardization.
Results were communicated at scientific meetings and published in scientific journals:
The results of this study were quoted in 1995 by a French group working on sorting of bovine chromosomes in Mammalian Genome (Schmitz et al.:415-420, The Bovine Bivariate Flow Karyotype and Peak Identification by Chromosome Painting with PCR-generated probes).
KLEBERG Fellow in Medical Genetics (molecular genetics & bioinformatics)
Section of Medical Genetics, Department of Clinical Studies, School of Veterinary Medicine, University of Pennsylvania 3900 Delancey St, Philadelphia PA 19104-6010 USA.
Director: Dr D.F. Patterson
Kleberg Fellowship awarded for three years, after international competition to veterinarians following a career in comparative genetics at the molecular and cellular levels.
I used various molecular genetics techniques to clone and sequence the gene coding for the enzyme Beta-Glucuronidase in the dog.
Analysis at the molecular level of inherited diseases in the dog is useful to detect carriers of these anomalies, characterization of homologous models to human genetic diseases, and use of these animals in gene therapy trials.
The gene for the enzyme Beta-Glucuronidase (GUSB) has been sequenced in man, mouse, rat and E. coli, and more than 25 mutations have been described in man, whereas only one mutation was reported in mouse, leading to the lysosomal disease Mucopolysaccharidosis type VII (MPSVII) or "Sly's Disease". Since clinical cases of canine MPSVII have been described , based on abnomal levels of Glycosaminoglycans (GAGs) in the urine and reduced levels of enzyme activity, these animals could provide a clinical model for gene therapy of MPSVII in humans. The first step requiring the cloning and sequencing of the normal gene before the mutation could be identified.
Using various molecular genetics techniques, cDNA clones for canine GUSB were isolated fom cDNA libraries and sequenced. After comparison with published human and murine cDNA GUSB sequences, it appears that the canine sequence is 80% identical to the human sequence (they have the same number of codons) whereas in mouse and rat, there are 76% identity with the human sequence (three codons are not present).
Results of these studies have permitted the characterization of the full-lenght normal sequence (deposited in GENEBANK), the detection of the mutation and the invitro correction of enzyme activity in MPSVII dog cell lines .
They have been presented at various meetings and published in scientific journals.
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19982-1988, Graduate Teaching Assistant, University of Guelph.
In different aspects:
I also followed the training sessions of the Office for Educational Practice of the University of Guelph:
During my stay at the Universite de Pennsylvania, I was included in the teaching of the Medical Genetics Course to the veterinary students and I was one of the moderators for their sessions of Problem-Based Learning in Veterinary Medical Genetics.
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PUBLIHELP, 12 rue Caillaux 75013 PARIS Tel: 01 44 06 07 08
PUBLIHELP is a medical information services organisation for industry ( pharmaceutical laboratories, Biotechnology groups, manufacturers and distributors of medical supplies) health sciences professionnals, Communication agencies, public institutions and the medical press.
My different fonctions include:
- Coordination of a team of internal and external translaters
The Medical and Scientific Translation Branch (TSM) involves the translation of various documents (scientific abstracts & articles, medical files, advertising, official texts, patients information sheets, software training manuals), vidéo tapes, web sites
- Specialised information searches & and bibliographic summaries for different clients
- Member of proofreading and validation committee for approval of documents before sending to customers
- Customer relations (recruitment of new clients, contacts, invoice negotiations, sendind documents, billing and follow-up)
-LAN (local area network) management
- Web pages writing and updating http://www.tsm-paris.fr, http://www.publihelp.fr
- Organisation of events (Congresses) :
Design and dissemination of congress announcement & programme
Participants Wellcome & Registration
Invitation, accomodation & transportation of invited speakers
HEAD, HOUSE of ORPHAN DISEASES
Association Française de Recherche Genetique
5 rue Casimir Delavigne
(Human medical genetics, project management, scientific communication, external relations and microinformatics).
My fonction implies scientific information and orientation in
response to requests from patients, their families, health
professionnals and the public. Mostly about rare genetic
diseases. In two years, we have received more than 3000
concerning more than 900 different diseases.
Through the Internet, I have established contacts with various support groups around the world and I am running the web site for the AFRG (http: //www.multimania.com/afrg). In a year, we received more than 12,000 visits and each month, 20% of the requests for information come through e-mail.
For the past three years, in collaboration with a pharmaceutical firm, I have edited a booklet on orphan genetic diseases to be distributed to general practitioners and pharmacists.
I have been in contacts with journalists from various medias (TV, Radio and the Press).
As secretary of the Scientific Council for the AFRG, I am also responsible for organizing the meetings, and the management of the research fellowships awarded by the AFRG. In two years we have awarded seven fellowships.
I have been involved in the organizing and running of round table talks and plenary sessions on the familial and social impacts of orphan genetic diseases and I have represented the AFRG at various scientific meetings including the 7th European Congress on Neurofibromatosis where I also delivered a talk ( The French House of Orphan Diseases, 7th European Congress on Neurofibromatosis, Paris, December 4-6), and the International Congress on Von Hippel-Lindau, Paris, September 16-18 1998.
On the international level, I have been involved in the EAGS (European Alliance of Genetic Support Groups) and I have participated at various meetings and human genetic congresses.
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I have regularly presented seminars at various scientific institutions in North America and Europe.
B) INTERNATIONAL RELATIONS
Since 1982, through my research, teaching and scientific communication activities in North America and Europe, my participation at scientific meetings and publications in scientific papers, I have regularly contributed to international exchange of information and cooperation.
I have been involved in written translation of manuals for specialized software packages, of annual reports, and of scientific papers, as well as in verbal translation during scientific meetings. I have also contributed to the Dictionnary of Animal Production Technology, Elsevier Amsterdam, 1993, ISBN 0-444-88072-0, (see acknowledgements page XX).
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SYNTHESIS OF EXPERIENCE and PLANNING FOR THE FUTURE
Since 1982, all my research and teaching activities have been concentrated in Biomedical Sciences field (the interface between fondamental biology and medicine). My training and experience in comparative molecular and medical genetics techniques, (manipulations of viral, bacterial and eukaroytic DNA), their applications in medicine, and in bioinformatics, will enable me to continue my career by studying portions of genes that are conserved throughout evolution and that have pharmacological significance.
Since I have worked and studied for more than 11 years in various English-speaking countries (Canada, the United Kingdom and the United States of America), I am fully bilingual and all my scientific publications are in English. I have established an international network of scientific contacts and I am particularly interested to continue to play a role in scientific communication at the international level. I am always willing to interact with people from various professions at various levels.
As a new step in my career, I have successfully been admitted to the qualification lists for the French University Professors and the Researchers for the Natural History Museum.
This unique combination of training in Biomedical Sciences, and international experience in biotechnology applied to human and animal health, will enable me to pursue a career in scientific information in the pharmaceutical industry.
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Management Other activities Future Directions